Multilayered Nanostructures Integrated with Emerging Technologies

Surface and interface functionalization are crucial steps to introduce new functionalities in numerous applications, as faster dynamics occur on surfaces rather than bulk. Within this context, the layer-by-layer (LbL) technique is a versatile methodology to controllably form organized nanostructures from the spontaneous adsorption of charged molecules. It enables the assembly of multilayered LbL films on virtually any surface using non-covalent molecular interactions, allowing the nanoengineering of interfaces and creation of multifunctional systems with distinct building blocks (polymers, clays, metal nanoparticles, enzymes, organic macromolecules, etc.). Several applications require thin films on electrodes for sensing/biosensing, and here we explore LbL films deposited on interdigitated electrodes (IDEs) that were 3D-printed using the fusing deposition modeling (FDM) technique. IDEs covered with LbL films can be used to form multisensory systems employed in the analysis of complex liquids transforming raw data into specific patterns easily recognized by computational and statistical methods. We extend the FDM 3D-printing methodology to simplify the manufacturing of electrodes and microchannels, thus integrating an e-tongue system in a microfluidic device. Moreover, the continuous flow within microchannels contributes to faster and more accurate analysis, reducing the amount of sample, waste, and costs

3D Printed e-Tongue

Nowadays, one of the biggest issues addressed to electronic sensor fabrication is the build-up of efficient electrodes as an alternative way to the expensive, complex and multistage processes required by traditional techniques. Printed electronics arises as an interesting alternative to fulfill this task due to the simplicity and speed to stamp electrodes on various surfaces. Within this context, the Fused Deposition Modeling 3D printing is an emerging, cost-effective and alternative technology to fabricate complex structures that potentiates several fields with more creative ideas and new materials for a rapid prototyping of devices. We show here the fabrication of interdigitated electrodes using a standard home-made CoreXY 3D printer using transparent and graphene-based PLA filaments. Macro 3D printed electrodes were easily assembled within 6 min with outstanding reproducibility. The electrodes were also functionalized with different nanostructured thin films via dip-coating Layer-by-Layer technique to develop a 3D printed e-tongue setup. As a proof of concept, the printed e-tongue was applied to soil analysis. A control soil sample was enriched with several macro-nutrients to the plants (N, P, K, S, Mg, and Ca) and the discrimination was done by electrical impedance spectroscopy of water solution of the soil samples. The data was analyzed by Principal Component Analysis and the 3D printed sensor distinguished clearly all enriched samples despite the complexity of the soil chemical composition. The 3D printed e-tongue successfully used in soil analysis encourages further investments in developing new sensory tools for precision agriculture and other fields exploiting the simplicity and flexibility offered by the 3D printing techniques

Relevamiento arquitectónico del sitio Masao, Santa María, Catamarca, con VANT

La presente ponencia tiene como objetivo presentar el trabajo de actualización del plano arqueológico del sitio Masao. Se trata de un poblado prehispánico ubicado en el departamento Santa María de la provincia de Catamarca, correspondiente al período de los Desarrollos Regionales. Atento a ello, realizamos un nuevo relevamiento planimétrico del sitio, utilizando un VANT-drone marca PHANTOM, modelo 4. El relevamiento se realizó mediante pilotaje manual con control visual directo desde el pie del cerro, cubriendo una distancias de 600 a 1200 metros. Se tomaron 158 fotografías verticales (fotogramas) con una superposición del 80% aprox., que fueron procesadas con elprograma AGISOFT para conformar el fotomosaico final, con una definición de 4,61 cm/pixel. Paralelamente se digitalizó y georreferenció el plano relevado por Weiser, el cual se superpuso a nuestro fotograma. La correspondencia entre ambos registros es muy buena para el sector relevado, atento a la diferencia tecnológica utilizada en ambos casos. Adicionalmente, se pudieron registrar un total de 73 estructuras que no habían sido previamente relevadas, totalizando 181 estructuras. De este modo podemos dar a conocer el primer plano completo de este sitio.Fil: Lanzelotti, Sonia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto de las Culturas. Universidad de Buenos Aires. Instituto de las Culturas; ArgentinaFil: Lamami, Marcelo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto de las Culturas. Universidad de Buenos Aires. Instituto de las Culturas; ArgentinaFil: Gaál, Erico Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto de las Culturas. Universidad de Buenos Aires. Instituto de las Culturas; ArgentinaFil: Cabrera, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Instituto de las Culturas. Universidad de Buenos Aires. Instituto de las Culturas; ArgentinaXX Congreso Nacional de Arqueología ArgentinaCórdobaArgentinaUniversidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Instituto de Antropología de CórdobaUniversidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Museo de AntropologíaUniversidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Carrera de Antropologí

Femtosecond laser micromachining of polylactic acid/graphene composites for designing interdigitated microelectrodes for sensor applications

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORMCTIC - MINISTÉRIO DA CIÊNCIA, TECNOLOGIA, INOVAÇÕES E COMUNICAÇÕESEMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIAThere is an increasing interest in the last years towards electronic applications of graphene-based materials and devices Fabricated from patterning techniques. with the ultimate goal of high performance and temporal resolution. Laser micromachining using femtosecond pulses is an attractive methodology to integrate graphene-based materials into functional devices as it allows changes to the focal volume with a submicrometer spatial resolution due to the efficient nonlinear nature of the absorption, yielding rapid prototyping for innovative applications. We present here the patterning of PLA-graphene films spin coated on a glass substrate using a fs-laser at moderate pulse energies to fabricate interdigitated electrodes having a minimum spatial resolution of 5 pm. Raman spectroscopy of the PLA-graphene films indicated the presence of multilayered graphene fibers. Subsequently, the PLA-graphene films were micromachined using a femtosecond laser oscillator delivering 50-fs pulses and 800 nm, where the pulse energy and scanning speed was varied in order to determine the optimum irradiation parameters (16 of and 100 mu m/s) to the fabrication of microstructures. The micromachined patterns were characterized by optical microscopy and submitted to electrical measurements in liquid samples, clearly distinguishing all tastes tested. Our results confirm the femtosecond laser micromachining technique as an interesting approach to efficiently pattern PLA-graphene filaments with high precision and minimal mechanical defects, allowing the easy fabrication of interdigitated structures and an alternative method to those produced by conventional photolithography.1017479FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORMCTIC - MINISTÉRIO DA CIÊNCIA, TECNOLOGIA, INOVAÇÕES E COMUNICAÇÕESEMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIAFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL E NÍVEL SUPERIORMCTIC - MINISTÉRIO DA CIÊNCIA, TECNOLOGIA, INOVAÇÕES E COMUNICAÇÕESEMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA2011/12399-02013/03487-82013/6122672014/16789-52015/13140-0sem informaçãosem informaçãosem informaçãosem informaçã

Susceptibility loci for intracranial aneurysm in European and Japanese populations

Stroke is the world's third leading cause of death. One cause of stroke, intracranial aneurysm, affects similar to 2% of the population and accounts for 500,000 hemorrhagic strokes annually in mid-life (median age 50), most often resulting in death or severe neurological impairment(1). The pathogenesis of intracranial aneurysm is unknown, and because catastrophic hemorrhage is commonly the first sign of disease, early identification is essential. We carried out a multistage genome-wide association study (GWAS) of Finnish, Dutch and Japanese cohorts including over 2,100 intracranial aneurysm cases and 8,000 controls. Genome-wide genotyping of the European cohorts and replication studies in the Japanese cohort identified common SNPs on chromosomes 2q, 8q and 9p that show significant association with intracranial aneurysm with odds ratios 1.24-1.36. The loci on 2q and 8q are new, whereas the 9p locus was previously found to be associated with arterial diseases, including intracranial aneurysm(2-5). Associated SNPs on 8q likely act via SOX17, which is required for formation and maintenance of endothelial cells(6-8), suggesting a role in development and repair of the vasculature; CDKN2A at 9p may have a similar role(9). These findings have implications for the pathophysiology, diagnosis and therapy of intracranial aneurysm

Five loci with a genome-wide significant association to saccular intracranial aneurysm (sIA) disease in the Finnish and Dutch samples.

*<p>For each variant minor allele/major allele, locus and base pair position are given.</p>**<p>The variant's distance (kb) to the nearest gene is given.</p>***<p>Located in the intron of the given gene.</p>†<p>The previously reported 9p21.3 locus <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004134#pgen.1004134-Yasuno2" target="_blank">[12]</a>, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004134#pgen.1004134-Helgadottir1" target="_blank">[39]</a>.</p>‡<p>The previously studied 2q33.3 locus with inconclusive evidence (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004134#s4" target="_blank">Materials and Methods</a>).</p>§<p>Some heterogeneity in effect sizes exists between cohorts. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004134#pgen.1004134.s015" target="_blank">Table S9</a> for heterogeneity statistics.</p

The Finnish and Dutch study samples used in the association analysis of saccular intracranial aneurysm (sIA) disease.

*<p>Unknown familial sIA status for 35 patients.</p>**<p>Number of sIAs not known for 16 patients.</p><p>SD = Standard deviation.</p

The locus with a genome-wide significant association to the number of saccular intracranial aneurysms (sIA) per individual in the Finnish samples.

*<p>For each variant major allele/minor allele, locus and base pair position are given.</p>**<p>Located in the intron of the given gene.</p>§<p>See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004134#pgen.1004134.s015" target="_blank">Table S9</a> for heterogeneity statistics.</p

Study design.

<p>The Finnish discovery and replication cohorts represent a population with over two-fold increased risk of subarachnoid hemorrhage from ruptured saccular intracranial aneurysm (sIA-SAH). The Finnish discovery cohort was intentionally enriched with familial sIA patients, and 9.4M genotyped and imputed variants were studied. The loci with p<5E-6 were replicated in an independent and unselected Finnish sIA sample. The allele frequencies and effect sizes of the replicated variants in Finland were finally compared to continental European population using a Dutch sample. The sIA-SAH risk is not increased in the Netherlands (‘general risk’ in the figure).</p